Freeze-Fracture Quiz

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Membrane fracture basics

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johnnycham

Malasia

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Froggy Jumps Freeze-Fracture QuizVersión en línea Membrane fracture basics por johnnycham 1 what is freeze fracture a Internal membrane architecture of cells b 3D cytoskeletal networks c Nuclear DNA sequences 2 During freeze-fracture, what is fractured? a The phospholipid bilayer of membranes b DNA strands c Protein complexes 3 What is created to study membranes in freeze-fracture? a A plastic mold of the nucleus b A metal-coated replica of the fracture surface c A dried biopsy smear 4 Why are cells rapidly frozen in liquid nitrogen? a To heat it for better slicing b To preserve structure by preventing ice crystal damage c To dry the sample for coating 5 outline the 3 steps to freeze fracturing a the cell fractures at the weakest point of the cell membrane, splitting it into two layers b proteins embedded within the membrane will remain one of the two layers c this procedure helps visualise into the surface of the cells 6 Which instrument is used to visualize freeze-fracture replicas? a Transmission electron microscope b Light microscope c Scanning electron microscope 7 Freeze-fracture is especially useful for studying what aspect of membranes? a DNA packaging in the nucleus b Lipid melting points c Distribution of membrane proteins 8 A common artifact in freeze-fracture is caused by what if cooling is not fast enough? a Fluorescence quenching b Ice crystal damage causing artifacts c Protein denaturation only

Froggy Jumps

Freeze-Fracture QuizVersión en línea

Membrane fracture basics

por johnnycham

what is freeze fracture

Internal membrane architecture of cells

3D cytoskeletal networks

Nuclear DNA sequences

During freeze-fracture, what is fractured?

The phospholipid bilayer of membranes

DNA strands

Protein complexes

What is created to study membranes in freeze-fracture?

A plastic mold of the nucleus

A metal-coated replica of the fracture surface

A dried biopsy smear

Why are cells rapidly frozen in liquid nitrogen?

To heat it for better slicing

To preserve structure by preventing ice crystal damage

To dry the sample for coating

outline the 3 steps to freeze fracturing

the cell fractures at the weakest point of the cell membrane, splitting it into two layers

proteins embedded within the membrane will remain one of the two layers

this procedure helps visualise into the surface of the cells

Which instrument is used to visualize freeze-fracture replicas?

Transmission electron microscope

Light microscope

Scanning electron microscope

Freeze-fracture is especially useful for studying what aspect of membranes?

DNA packaging in the nucleus

Lipid melting points

Distribution of membrane proteins

A common artifact in freeze-fracture is caused by what if cooling is not fast enough?

Fluorescence quenching

Ice crystal damage causing artifacts

Protein denaturation only

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Freeze-Fracture Quiz

Froggy Jumps

Sobre esta actividad

Creada por

Descarga la versión para jugar en papel

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Froggy Jumps

Freeze-Fracture QuizVersión en línea

por johnnycham

what is freeze fracture

During freeze-fracture, what is fractured?

What is created to study membranes in freeze-fracture?

Why are cells rapidly frozen in liquid nitrogen?

outline the 3 steps to freeze fracturing

Which instrument is used to visualize freeze-fracture replicas?

Freeze-fracture is especially useful for studying what aspect of membranes?

A common artifact in freeze-fracture is caused by what if cooling is not fast enough?

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