Antigen-Antibody Insights -II Quiz

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Challenge your knowledge of immunoassays and antigen–antibody tests.

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Quader Ahmed Jalili

India

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Antigen-Antibody Insights -II Quiz


Froggy Jumps Antigen-Antibody Insights -II QuizVersión en línea Challenge your knowledge of immunoassays and antigen–antibody tests. por Quader Ahmed Jalili 1 What does ELISA stand for? a Enzyme-Linked Immunoassay b Enzyme-Linked Immunosorbent Assay c Enzyme-Linked Immunoassay System 2 Which ELISA type detects antigens in serum using a labeled primary antibody? a Direct ELISA b Indirect ELISA c Sandwich ELISA 3 In Sandwich ELISA, what forms the sandwich? a Two capture antibodies and antigen b Capture antibody, antigen, detector antibody c Antigen, enzyme, substrate 4 What is the main advantage of ELISA in large labs? a No equipment needed b High throughput with 96-well plates c Single-sample precision 5 Which ELISA variant uses anti-human IgM to capture IgM? a Indirect ELISA b Direct ELISA c IgM Antibody Capture (MAC) ELISA 6 What is the principle of competitive ELISA? a No color change b Color inversely proportional to antigen amount c Color directly proportional to antigen amount 7 Which immunoassay is often automated and fluorescence-based? a CLIA b ELFA c Western blot 8 What does CLIA stand for? a Cartridge-Linked Immunoassay b Chemiluminescence Immunoassay c Cardiolipin Immunoassay 9 Which assay uses luminescence detection for higher sensitivity? a ELISA b IF A c CLIA 10 Western blot is primarily used for what? a Detect antibodies to specific proteins b Culture pathogens c Quantify antigen directly 11 What does IFA detect? a Colorimetric substrates only b Radioactive labels only c Antigens or antibodies using fluorescent dyes 12 Direct Immunofluorescence Assay uses which component for labelling? a Enzyme-labeled secondary antibody b Fluorescent dye-labeled primary antibody c Colloidal gold 13 Indirect Immunofluorescence Assay detects what? a Enzyme activity b Antigens in the serum c Antibodies in the serum 14 What is ELISA’s main detection readout? a Luminescence readout only b Fluorescence readout c Color change measured spectrophotometrically 15 ELISPOT is used to quantify which cells? a Surface antigens only b Cells producing antibodies or cytokines c Membrane-bound enzymes 16 IgG avidity ELISA helps differentiate which infection stage? a Recent vs past infection b Acute vs chronic c Viral load vs immunity 17 Which technique uses laser beam for cell analysis, ex: CD4 T cells? a ELISA b Flow Cytometry c Western blot 18 What is the main goal of lateral flow immunochromatographic tests? a Laboratory ELISA replacement b Rapid, point-of-care antigen/antibody detection c Quantitative tissue culture 19 In ICT, what marks the test line at the membrane? a Colorless plain membrane b Monoclonal antibody–antigen complex c Colloidal gold alone 20 What constitutes the control line in ICT rapid tests? a Antibody tagged with colloidal gold b Monoclonal antibody c Anti-species (Human) immunoglobulin 21 What does a positive ELISA indicate in screening? a Absolute proof of disease b No clinical relevance c Presence of antigen or antibody detected 22 Which assay uses Protein A to detect HIV antibodies among rapid tests? a Flow-through assay b IgG avidity ELISA c MAC ELISA 23 What is a key disadvantage of rapid tests? a Low sensitivity and specificity b Too slow results c Need specialized labs 24 What is a main advantage of ELISA over rapid tests? a No equipment required b Only qualitative results c Quantitative results possible 25 Which test confirms ELISA results due to higher specificity? a Western blot b MAC ELISA c ELISA alone 26 What detection method does IFA primarily use? a Fluorescent signal b Radioactivity c Enzymatic color 27 Which assay is commonly used for Latent TB diagnosis via IFN-γ? a CLIA b Western blot c ELISPOT 28 What does a ‘sandwich’ ELISA require precoated on the well? a Antigen only b Capture antibody c Detector antibody 29 Which antigen–antibody technique can yield a color change via chromogen? a Western blot b ELISA c Flow cytometry 30 In MAC ELISA, what is pre-coated on the well? a Recombinant antigen b Anti-human IgM c Secondary Ab- Biotin conjugate 31 Which method detects multiple antibodies against different antigenic fragments separated by gel electrophoresis? a Western blot b MAC ELISA c ELISA 32 Which assay uses a nitrocellulose membrane for readout? a Immunochromatographic lateral flow b Flow cytometry c ELISA plate 33 What is a common use of ELISA in infectious diseases? a Antigen or antibody detection b Gene sequencing c Bacterial culture 34 What does a positive HIV TRIDOT flow-through test show? a HIV antigens only b Antibodies to HIV-1/2 c No antibodies detected 35 What instrument reads ELISA color changes? a Flow cytometer b ELISA reader c Fluorometer 36 Which test is best for differentiating recent from past infections? a Western blot b CLIA c IgG avidity ELISA

Antigen-Antibody Insights -II Quiz

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Antigen-Antibody Insights -II QuizVersión en línea

por Quader Ahmed Jalili

What does ELISA stand for?

Which ELISA type detects antigens in serum using a labeled primary antibody?

In Sandwich ELISA, what forms the sandwich?

What is the main advantage of ELISA in large labs?

Which ELISA variant uses anti-human IgM to capture IgM?

What is the principle of competitive ELISA?

Which immunoassay is often automated and fluorescence-based?

What does CLIA stand for?

Which assay uses luminescence detection for higher sensitivity?

Western blot is primarily used for what?

What does IFA detect?

Direct Immunofluorescence Assay uses which component for labelling?

Indirect Immunofluorescence Assay detects what?

What is ELISA’s main detection readout?

ELISPOT is used to quantify which cells?

IgG avidity ELISA helps differentiate which infection stage?

Which technique uses laser beam for cell analysis, ex: CD4 T cells?

What is the main goal of lateral flow immunochromatographic tests?

In ICT, what marks the test line at the membrane?

What constitutes the control line in ICT rapid tests?

What does a positive ELISA indicate in screening?

Which assay uses Protein A to detect HIV antibodies among rapid tests?

What is a key disadvantage of rapid tests?

What is a main advantage of ELISA over rapid tests?

Which test confirms ELISA results due to higher specificity?

What detection method does IFA primarily use?

Which assay is commonly used for Latent TB diagnosis via IFN-γ?

What does a ‘sandwich’ ELISA require precoated on the well?

Which antigen–antibody technique can yield a color change via chromogen?

In MAC ELISA, what is pre-coated on the well?

Which method detects multiple antibodies against different antigenic fragments separated by gel electrophoresis?

Which assay uses a nitrocellulose membrane for readout?

What is a common use of ELISA in infectious diseases?

What does a positive HIV TRIDOT flow-through test show?

What instrument reads ELISA color changes?

Which test is best for differentiating recent from past infections?