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Gen Micro

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Part 2

por Consistency
1

Which of the following indicates a positive presumptive presence of Salmonella using Xylose Lysine Deoxycholate agar after incubation at 37°C for 24hrs?

2

What is the purpose of using a selective agar in microbial plating?

3

What is the purpose of staining a biological sample before observing it under a microscope?

4

Ernst August Friedrich Ruska was a German physicist who won the Nobel Prize in Physics in 1986. He is known to have invented the first_________.

5

What is the acceptable range of viable counts per plate, as specified by the Bacteriological Analytical Manual (BAM), for calculating colony-forming units for yeast and molds?

6

Penicillin blocks cell wall synthesis. During which phase of the growth cycle would a population of Streptococcus be most sensitive to penicillin?

7

How does non-ionizing radiation exert its antimicrobial effect?

8

Salmonella has a generation time of 30minutes. If four cells are present on a turkey that is left on the counter, how many cells will be present after 3hours?

9

Which colony appearance is most typical of Salmonella on Brilliant Green Agar?

10

A bacterial population increases from 10^3 cells to 10^8 cells in 6hours. How many generations have passed? Calculate for the growth rate.

11

A bacterial population increases from 10^3 cells to 10^8 cells in 6hours. What is the doubling time?

12

What is the color of the cells containing endospores after staining?

13

The following results to a pink/red stain, except for:

14

A food microbiologist observes yellow colonies surrounded by yellow zones on Mannitol Salt Agar (MSA). Which interpretation is most appropriate?

15

Based on the Bacteriological Analytical Manual (BAM), what is the set valid count range per plate for computing the Colony Forming Units for Aerobic plate count?

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BAM Chapter 18: Yeast, Molds, and Mycotoxins (10-150 colonies)

During log phase, bacteria actively divide and synthesize new cell walls, making them high sensitive to penicillin.

Non-ionizing radiation, such as ultraviolet (UV) light, exerts its anitmicrobial effect by damaging microbial DNA, specifically through formation of pyramidine dimers (thymine-thymine bonds).

G = 30 mins N1 = 4 cells t= 3 hours there is 6 30 minutes in 3 hours Sol. N2=4x2x2x2x2x2x2= 256cells

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