Basics of Molecular Biology & Sequencing

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nora shebl

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Basics of Molecular Biology & Sequencing


Basics of Molecular Biology & SequencingVersión en línea Molecular biology quiz por nora shebl 1 Which three biomolecule types are central to cellular function? a Lipids b Nucleotides c Carbohydrates d DNA, RNA, and proteins 2 What is the flow of genetic information called in biology? a RNA -> DNA -> Protein b DNA -> Protein -> RNA c DNA -> RNA -> Protein (central dogma) d Protein -> RNA -> DNA 3 Which enzyme copies DNA during replication? a Reverse transcriptase b DNA polymerase c Ligase d RNA polymerase 4 What is a codon? a A type of RNA b A protein domain c A single nucleotide mutation d A trinucleotide that encodes an amino acid 5 How do prokaryotes and eukaryotes differ in DNA organization? a Eukaryotes have no DNA b Prokaryotes have a single circular chromosome; eukaryotes have linear chromosomes in the nucleus c Both have circular chromosomes d Both have multiple linear chromosomes 6 Which sequencing method uses ddNTPs to terminate DNA synthesis? a SOLiD sequencing b Illumina sequencing c Roche/454 sequencing d Sanger sequencing 7 What read length is typical for Illumina sequencing platforms? a 1000 bases b Around 35–50 bases per read c 10 bases d 200–300 bases 8 Why are long repeats a problem for genome assembly? a They improve assembly accuracy b They do not affect assembly at all c They always occur in noncoding regions d If repeat length exceeds read length, overlaps are unclear 9 What is BAC-by-BAC sequencing? a Sequence the whole genome in one run b Ignore repeats with BACs c Use BACs only for annotation d Divide genome into ~150 kb BACs and sequence each separately 10 What is a contig in sequence assembly? a A single read without overlaps b A cloned plasmid c A gene with multiple exons d A contiguous sequence formed by overlapping reads Feedback 1 DNA stores information, RNA transfers/ templates, proteins do the work. 2 Transcription transfers info from DNA to RNA, translation makes protein. 3 DNA polymerase synthesizes new DNA strands using a template. 4 Triplets encode amino acids; there are 64 possible codons. 5 Prokaryotic DNA is typically a circular chromosome; eukaryotic DNA is linear and in nucleus. 6 Sanger (dideoxy) sequencing uses chain-terminating ddNTPs. 7 Illumina reads are short, usually tens of bases long per read. 8 When repeats longer than reads exist, correct placement is hard. 9 Each BAC is sequenced; assembly then merges BAC contigs. 10 Contigs result from merging reads that overlap to form longer sequences.

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Basics of Molecular Biology & SequencingVersión en línea

por nora shebl

Which three biomolecule types are central to cellular function?

What is the flow of genetic information called in biology?

Which enzyme copies DNA during replication?

What is a codon?

How do prokaryotes and eukaryotes differ in DNA organization?

Which sequencing method uses ddNTPs to terminate DNA synthesis?

What read length is typical for Illumina sequencing platforms?

Why are long repeats a problem for genome assembly?

What is BAC-by-BAC sequencing?

What is a contig in sequence assembly?

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