Gene Expression Challenge

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Test your knowledge of transcription, translation, and regulation

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Isabelle Shiraishi

Estados Unidos

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Gene Expression Challenge


Gene Expression ChallengeVersión en línea Test your knowledge of transcription, translation, and regulation por Isabelle Shiraishi 1 Which promoter elements are typical for prokaryotes? a CAAT box and GC box b TATA box and -25 region c TATA box and Inr element d -10 Pribnow box and -35 sequences 2 Which core promoter element is characteristic of eukaryotic transcription? a CAAT box at -75 b -25 GC box c TATA box at -29 (with RNA Pol II) d Pribnow box (-10) 3 What happens when LacI repressor binds the lac operator (O)? a RNA processing is initiated b Ribosome binding is blocked c Transcription of lacZ/Y/A is inhibited d Transcription is enhanced 4 In the presence of lactose, what acts as the inducer to relieve LacI repression? a Cyclic AMP b Lactose-6-phosphate c Glucose d Allolactose (IPTG in lab) 5 What regulatory mechanism uses a leader peptide to terminate transcription in some cells? a Attenuation (trp operon) b Phosphorylation of RNA polymerase c DNA methylation d RNA editing 6 Which molecular chaperones are commonly involved in protein folding? a Proteasome subunits only b Hsp70, Hsp40, GroEL/GroES, Hsp90 c Ribosome recycling factors d DNA polymerase and ligase 7 Name a common post-translational modification listed in the slides. a Ubiquitination b Glycolysis tagging c DNA phosphorylation d RNA methylation 8 What is the insulin processing pathway as described? a Preproinsulin → Proinsulin → Insulin (with disulfide formation) b Insulin → Proinsulin → Preproinsulin c Preproinsulin → Insulin → Proinsulin d Proinsulin → Insulin → Preproinsulin 9 What is meant by “alternative” promoters in gene regulation? a Promoters only for developmental genes b Multiple promoters for different times or cell types c A single promoter with variable strength d Promoters that always activate equally in all cells 10 Which regulator provides catabolite repression of the lac operon? a CRP-cAMP complex b LacI repressor c Sigma factor S d TTGACA factor Feedback 1 Prokaryotic promoters commonly have a -10 Pribnow box and -35 region. 2 Eukaryotic core promoters often include a TATA box recognized by TBP. 3 LacI binding to O represses transcription of lac operon genes. 4 Allolactose binds LacI causing dissociation from the operator; IPTG is a lab inducer. 5 Attenuation in the trp operon uses a leader peptide to terminate transcription under certain tryptophan levels. 6 Chaperones like Hsp70/Hsp40 and GroEL/GroES assist folding; Hsp90 also participates. 7 Post-translational modifications include ubiquitination among others. 8 Preproinsulin is processed to proinsulin, then to active insulin with disulfide bonds. 9 Alternative promoters provide tissue- or time-specific expression. 10 CRP-cAMP binding stimulates transcription when glucose is low, relieving repression.

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Gene Expression Challenge

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Creada por

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Gene Expression ChallengeVersión en línea

por Isabelle Shiraishi

Which promoter elements are typical for prokaryotes?

Which core promoter element is characteristic of eukaryotic transcription?

What happens when LacI repressor binds the lac operator (O)?

In the presence of lactose, what acts as the inducer to relieve LacI repression?

What regulatory mechanism uses a leader peptide to terminate transcription in some cells?

Which molecular chaperones are commonly involved in protein folding?

Name a common post-translational modification listed in the slides.

What is the insulin processing pathway as described?

What is meant by “alternative” promoters in gene regulation?

Which regulator provides catabolite repression of the lac operon?

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