Genetic Engineering Essentials

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Basics of GMOs and enzymes

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Haniya Ahmed

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Genetic Engineering Essentials


Genetic Engineering EssentialsVersión en línea Basics of GMOs and enzymes por Haniya Ahmed 1 What do restriction enzymes typically produce when cutting DNA for gene transfer? a Overlapping loops b Blunt ends c Floating ends d Sticky ends 2 Which enzyme seals together the transferred gene into the plasmid? a Reverse transcriptase b DNA helicase c DNA ligase d RNA polymerase 3 What is a plasmid in bacterial genetic engineering? a A bacterial ribosome b A linear chromosome c A protein enzyme d A circular DNA vector 4 Which organism is commonly used to produce human insulin in GM techniques? a Yeast b Human cells c Bacteria d Fungi 5 Why are restriction enzymes chosen carefully in gene cloning? a They cut everywhere b They degrade RNA c They cut at specific DNA sequences d They ligate DNA on contact 6 What happens after a recombinant plasmid is introduced into bacteria? a Bacteria replicate the plasmid b Plasmids are expelled from the cell c Plasmids disable the bacteria d Cells burst immediately 7 Transgenic organisms are GMOs that contain DNA from what source? a Only artificial DNA b Only viral DNA c A different species d The same species only 8 What is a potential advantage of GM crops? a Increased water use b Lower yields c Increased pest resistance d More seeds required 9 What is a common concern about GM crops? a They grow without soil b They are always unsafe c They cannot be sterile d Impact on biodiversity 10 Which statement is true about GMOs and transgenic organisms? a All transgenic organisms are GMOs, but not all GMOs are transgenic b Transgenic means identical species only c All GMOs are transgenic d GMOs cannot be transgenic Feedback 1 Sticky ends are short single-stranded overhangs that pair via complementary bases. 2 DNA ligase forms a covalent bond between DNA fragments. 3 Plasmids carry DNA and can be introduced into bacteria as vectors. 4 Bacteria are cultured in fermenters to produce insulin. 5 Specific enzymes cut at defined sequences creating compatible ends. 6 As bacteria divide, plasmids are copied and propagated. 7 Transgenic means DNA from a different species is introduced. 8 GM crops can carry pest or herbicide resistance genes. 9 Gene flow may affect wild species and ecosystems. 10 Transgenic refers to DNA from a different species; not all GMOs are transgenic.

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Genetic Engineering Essentials

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Genetic Engineering EssentialsVersión en línea

por Haniya Ahmed

What do restriction enzymes typically produce when cutting DNA for gene transfer?

Which enzyme seals together the transferred gene into the plasmid?

What is a plasmid in bacterial genetic engineering?

Which organism is commonly used to produce human insulin in GM techniques?

Why are restriction enzymes chosen carefully in gene cloning?

What happens after a recombinant plasmid is introduced into bacteria?

Transgenic organisms are GMOs that contain DNA from what source?

What is a potential advantage of GM crops?

What is a common concern about GM crops?

Which statement is true about GMOs and transgenic organisms?

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