Subcellular Fractionation Quiz

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Cell component separation basics

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Dr.Ruby Varghese

India

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Subcellular Fractionation Quiz


Subcellular Fractionation QuizVersión en línea Cell component separation basics por Dr.Ruby Varghese 1 Which centrifugation method is primarily used for this separation? a Gradient centrifugation b Differential centrifugation c Analytical ultracentrifugation d Isopycnic centrifugation 2 Name one property exploited to separate organelles. a Buoyant density b Thermal conductivity c Magnetic susceptibility d Electrical conductivity 3 Which condition is typically used during subcellular fractionation? a 4°C b 25°C c 37°C d -20°C 4 Which media is commonly used in differential centrifugation? a Sodium chloride b Sucrose c Ethanol d Acetone 5 Which of the following is NOT a property used for separation? a Buoyant density b Color c Surface charge density d Size and shape 6 Which other media may be used besides sucrose in differential centrifugation? a Glucose b Urea c Mannitol d NaCl 7 What property is not typically used as a separation parameter? a Surface charge density b Size and shape c Thermal conductivity d Buoyant density 8 Ficoll 400 is mentioned as a medium; what is its role? a React with proteins to label them b Provide high viscosity for separation c Calibrate pH d Act as a detergent 9 Why is the process done at high viscosity media? a To color the components b To increase temperature sensitivity c To denature proteins d To improve separation resolution 10 Which centrifugation method separates by size and yields crude fractions as the first step in fractionation? a Density gradient centrifugation b Rate zonal centrifugation c Differential centrifugation d Isopycnic centrifugation 11 Which interactions are disrupted to solubilize proteins? a Ionic bonds only b Hydrogen bonds, disulfide bonds, and hydrophobic interactions c Van der Waals forces only d Covalent peptide bonds 12 What is exposed when solubilizing hydrophobic regions? a Hydrophilic regions are created b Protein primary structure is degraded c Hydrophobic areas are exposed to the solvent d Disulfide bonds are formed 13 Which factor guides the solubilization method? a Only solvent viscosity b Only the pH of solvent c Protein properties like size, charge, and hydrophobicity d Temperature alone 14 Name a common solubilization agent? a Nucleotides b Detergents c Monosaccharides d Enzymes 15 Which class of agents disrupts hydrogen bonds non-specifically? a Buffer salts b Reducing agents c Chaotropic agents d Salts at low concentration 16 Which approach is used to solubilize proteins with high hydrophobicity? a Extreme cold only b Organic solvents c Aqueous buffers only d Strong acids 17 What is a typical role of detergents in solubilization? a Break protein-protein interactions and micellize hydrophobic regions b Hydrolyze peptide bonds c Protect proteins from all solvents d Form covalent bonds with proteins 18 Why might a salt aid solubilization? a They covalently modify amino acids b They always denature proteins c Salts can shield charges and reduce aggregation d They digest proteins 19 Which method is unsuitable for all proteins? a Detergent-based solubilization b Organic solvent solubilization c One-size-fits-all method d Chaotropic-based solubilization 20 What should be considered when selecting a solubilization method? a Protein size, charge, and hydrophobicity b Time of day c Color of solution d Protein name length Feedback 1 Differential centrifugation separates components by size and density in steps. 2 Buoyant density differences allow sequential pelleting of components. 3 Low temperature preserves organelle integrity and enzyme activity. 4 Sucrose solutions provide controlled density for gradient/viscosity adjustments. 5 Color is not a physical property used for fractionation. 6 Mannitol is a common osmotic stabilizer in fractionation protocols. 7 Thermal conductivity is not a standard parameter for organelle separation. 8 Ficoll 400 increases medium viscosity to aid differential separation. 9 Higher viscosity helps achieve sharper separations during centrifugation. 10 Differential uses size-based sedimentation to fractionate cells/organelles; density-based methods separate by density rather than crude size fractions. 11 Solubilization targets non-covalent protein interactions that hold structure together. 12 Solubilizing often involves exposing hydrophobic cores to solvent to disrupt aggregation. 13 Choice depends on the protein’s size, charge, and hydrophobicity. 14 Detergents are widely used to disrupt hydrophobic interactions. 15 Chaotropes destabilize structured water and macromolecular interactions. 16 Organic solvents can better dissolve highly hydrophobic proteins. 17 Detergents form micelles around hydrophobic regions to keep proteins soluble. 18 Salts modulate electrostatic interactions, aiding solubility in many cases. 19 No universal method; choice depends on protein properties. 20 Matching method to protein properties improves solubility and yield.

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Subcellular Fractionation Quiz

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Subcellular Fractionation QuizVersión en línea

por Dr.Ruby Varghese

Which centrifugation method is primarily used for this separation?

Name one property exploited to separate organelles.

Which condition is typically used during subcellular fractionation?

Which media is commonly used in differential centrifugation?

Which of the following is NOT a property used for separation?

Which other media may be used besides sucrose in differential centrifugation?

What property is not typically used as a separation parameter?

Ficoll 400 is mentioned as a medium; what is its role?

Why is the process done at high viscosity media?

Which centrifugation method separates by size and yields crude fractions as the first step in fractionation?

Which interactions are disrupted to solubilize proteins?

What is exposed when solubilizing hydrophobic regions?

Which factor guides the solubilization method?

Name a common solubilization agent?

Which class of agents disrupts hydrogen bonds non-specifically?

Which approach is used to solubilize proteins with high hydrophobicity?

What is a typical role of detergents in solubilization?

Why might a salt aid solubilization?

Which method is unsuitable for all proteins?

What should be considered when selecting a solubilization method?

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