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OPEN UP 5 UNIT 3: THERE IS/ARE, THERE WAS, THERE WERE

karen blixen

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eng genètica 3r


eng genètica 3rVersión en línea biotec por Mariona Miquel Garcia 1 Different restriction enzymes can generate the same sticky ends Sí No 2 The northern blot is a technique used to study ene expression by detection of DNA in a sample Sí No 3 Cell extracts used for in vitro translation provide ribosomes and tRNA Sí No 4 Multiplex-PCR uses one pair of primers annealing to diûerent target sequences Sí No 5 Haripins in primer loop are important for a correct binding to target DNA sequence Sí No 6 The yeast two-hybrid system is very useful but it does not allow to identify protein-protein interactions Sí No 7 .The Gateway cloning technology is based on site-speciûc recombination system Sí No 8 The substrate for DNA packaging in lambda particles ic circular DNA Sí No 9 All restriction endonucleases recognize hexanucleotide target sites and has a palindromic sequence Sí No 10 Phages derived from f1 that display a fusion protein in their surface cannot be generated so that they contain a genome with the DNA sequence that codes for that particular fusion protein Sí No 11 When working with lambda, replacement vectors accept less DNA than the insertion vectors Sí No 12 In situ hybridization allows you to locate a fragment of nucleic acid whitin a histologic section Sí No 13 It is generally accepted that the optimal length of PCR product is between 18-22 bp Sí No 14 Sticky-end ligation is more eûcient than blunt-end ligation Sí No 15 When designing a primer to introduce a change of amino acid in a protein, the mutation or mutations is/are in the center of the mutagenic primer Sí No 16 The hybridization probe for primer extension is a synthesized radio labeled oligonucleotide Sí No 17 At 94oC, DNA denaturates and strands are separated Sí No 18 In the Northern blot RNA is transferred from the electrophoresis agarose gel to the blotting nitrocellulose membrane Sí No 19 Genomic libraries are constructed using cDNA Sí No 20 In a PCR is always necessary to use degenerate primers Sí No 21 Oligo(dT) primers are used for cDNA synthesis reactions because of their speciûcity for mRNA Sí No 22 In RNase protection assay you can only detect one RNA in each reaction Sí No 23 Northern blot could be used to detect expression of a speciûc gene in diûerent tissues Sí No 24 Polymerases requires a primer to synthese new DNA Sí No 25 The gen called lacZ, encodes for beta-galactosidase, the enzyme involved in the breakdown of X-gal that give a deep blue colored product Sí No 26 PCR can be used for analyzing genotype or mouse models Sí No 27 In real time PCR a house keeping gene is used as an internal control Sí No 28 The product of lambda particles containing a cosmic genome requires the lysis of infected cells that contain such genome Sí No 29 RNA seq uses next-generation sequencing to quantify DNA in a sample at a precise moment in time Sí No 30 Housekeeping gene has diûerent expression in normal or pathological conditions Sí No 31 Inducible promoters allow the regulation of the expression of particular cDNAs placed under their control Sí No 32 To sequence the entire genome of an organism the most appropriate method is the Sanger method Sí No 33 The quick-change mutagenesis kit relies in methylation of the sequence GATC in E.coli and the lack of such modiûcation in newly in vitro-synthesized DNA when using PCR Sí No 34 The Sanger method relies on the Watson and Crick base pairing Sí No 35 There exist several technological platforms for high-throughput sequencing. Depending on your needs one platform may be more appropriate than the other Sí No 36 Linkers are attached to the end of sticky DNA molecule and can be cut to generate blunt ends Sí No 37 Klenow fragment can generate a new strand of DNA from an RNA template Sí No 38 Lambda can undertake the lysogenic cycle only when the genome is recombinant Sí No 39 Random priming is a good method to get speciûc labelling of one of the two DNA strands Sí No 40 To identify recombinant colonies, one strategy is to replica plated onto agar medium that contains a second antibiotic, for instance, tetracycline Sí No 41 In RACE PCR you can amplify a full length sequence of an RNA transcript Sí No 42 Polynucleotide kinase dephosphorilates 39-OH ends of DNA Sí No 43 mRNA is easily puriûed from total RNA using oligodC aûnity columns Sí No 44 When producing a new recombinant protein, independently of your goals, you must always add an aûnity tag Sí No 45 HIgher number of genes are detected in RNA seq versus Microarrays Sí No 46 RT-PCR allows you to compare gene expression between samples Sí No 47 Inducible promoters allow the regulation of the expression of particular cDNAs placed under their control Sí No 48 Asymmetric PCR uses excess of only one primer and preferentially ampliûes one strand of the target DNA Sí No 49 Endonucleases removes nucleotides from the end of a DNA molecule Sí No 50 In Real Time PCR we always use ûuorescent reporters to follow ampliûcation process Sí No 51 An expression cassette consists on a promoter the coding sequences for a particular protein and a transcription termination signal Sí No 52 Cosmids are plasmids with an special module that permits the packaging of recombinant genomes into lambda particles Sí No 53 Choosing the appropriate homology arms allows precise integration of DNA sequences in the yeast genome Sí No 54 The signal peptide that is often included when producing recombinant proteins in Piccia pastors has the role of targeting the recombinant protein to the micocondria where it can accumulate withot tox Sí No 55 Microarrays can be used to answer this question: Sí No 56 Depending on your goals or needs, cDNA libraries are prepared in diûerent vectors Sí No

eng genètica 3r

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Sí o No

OPEN UP 5 UNIT 3: THERE IS/ARE, THERE WAS, THERE WERE

Sí o No

¿Es verdad?

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Preguntas sobre futbol para niños

Sí o No

Grammys a mejor canción/grabación

Sí o No

¿Está creado con Inteligencia Artificial?

eng genètica 3rVersión en línea

por Mariona Miquel Garcia

Different restriction enzymes can generate the same sticky ends

The northern blot is a technique used to study ene expression by detection of DNA in a sample

Cell extracts used for in vitro translation provide ribosomes and tRNA

Multiplex-PCR uses one pair of primers annealing to diûerent target sequences

Haripins in primer loop are important for a correct binding to target DNA sequence

The yeast two-hybrid system is very useful but it does not allow to identify protein-protein interactions

.The Gateway cloning technology is based on site-speciûc recombination system

The substrate for DNA packaging in lambda particles ic circular DNA

All restriction endonucleases recognize hexanucleotide target sites and has a palindromic sequence

Phages derived from f1 that display a fusion protein in their surface cannot be generated so that they contain a genome with the DNA sequence that codes for that particular fusion protein

When working with lambda, replacement vectors accept less DNA than the insertion vectors

In situ hybridization allows you to locate a fragment of nucleic acid whitin a histologic section

It is generally accepted that the optimal length of PCR product is between 18-22 bp

Sticky-end ligation is more eûcient than blunt-end ligation

When designing a primer to introduce a change of amino acid in a protein, the mutation or mutations is/are in the center of the mutagenic primer

The hybridization probe for primer extension is a synthesized radio labeled oligonucleotide

At 94oC, DNA denaturates and strands are separated

In the Northern blot RNA is transferred from the electrophoresis agarose gel to the blotting nitrocellulose membrane

Genomic libraries are constructed using cDNA

In a PCR is always necessary to use degenerate primers

Oligo(dT) primers are used for cDNA synthesis reactions because of their speciûcity for mRNA

In RNase protection assay you can only detect one RNA in each reaction

Northern blot could be used to detect expression of a speciûc gene in diûerent tissues

Polymerases requires a primer to synthese new DNA

The gen called lacZ, encodes for beta-galactosidase, the enzyme involved in the breakdown of X-gal that give a deep blue colored product

PCR can be used for analyzing genotype or mouse models

In real time PCR a house keeping gene is used as an internal control

The product of lambda particles containing a cosmic genome requires the lysis of infected cells that contain such genome

RNA seq uses next-generation sequencing to quantify DNA in a sample at a precise moment in time

Housekeeping gene has diûerent expression in normal or pathological conditions

Inducible promoters allow the regulation of the expression of particular cDNAs placed under their control

To sequence the entire genome of an organism the most appropriate method is the Sanger method

The quick-change mutagenesis kit relies in methylation of the sequence GATC in E.coli and the lack of such modiûcation in newly in vitro-synthesized DNA when using PCR

The Sanger method relies on the Watson and Crick base pairing

There exist several technological platforms for high-throughput sequencing. Depending on your needs one platform may be more appropriate than the other

Linkers are attached to the end of sticky DNA molecule and can be cut to generate blunt ends

Klenow fragment can generate a new strand of DNA from an RNA template

Lambda can undertake the lysogenic cycle only when the genome is recombinant

Random priming is a good method to get speciûc labelling of one of the two DNA strands

To identify recombinant colonies, one strategy is to replica plated onto agar medium that contains a second antibiotic, for instance, tetracycline

In RACE PCR you can amplify a full length sequence of an RNA transcript

Polynucleotide kinase dephosphorilates 39-OH ends of DNA

mRNA is easily puriûed from total RNA using oligodC aûnity columns

When producing a new recombinant protein, independently of your goals, you must always add an aûnity tag

HIgher number of genes are detected in RNA seq versus Microarrays

RT-PCR allows you to compare gene expression between samples

Inducible promoters allow the regulation of the expression of particular cDNAs placed under their control

Asymmetric PCR uses excess of only one primer and preferentially ampliûes one strand of the target DNA

Endonucleases removes nucleotides from the end of a DNA molecule

In Real Time PCR we always use ûuorescent reporters to follow ampliûcation process

An expression cassette consists on a promoter the coding sequences for a particular protein and a transcription termination signal

Cosmids are plasmids with an special module that permits the packaging of recombinant genomes into lambda particles

Choosing the appropriate homology arms allows precise integration of DNA sequences in the yeast genome

The signal peptide that is often included when producing recombinant proteins in Piccia pastors has the role of targeting the recombinant protein to the micocondria where it can accumulate withot tox

Microarrays can be used to answer this question:

Depending on your goals or needs, cDNA libraries are prepared in diûerent vectors