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Unit 3 with PCR and Gel Electrophoresis

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Chloe Schneiderman

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Bio Study Guide


Bio Study GuideVersión en línea Unit 3 with PCR and Gel Electrophoresis por Chloe Schneiderman 1 What are Flanking Sequences a Sequences that DON'T repeat before STR b Sequences that DO repeat before STR c Sequences that DO repeat after STR d Sequences that DON'T repeat after STR 2 STRS Choose one or more answers a Long Nucleotide Length b Short Nucleotide Length c Long Tandem Repeats d Short Tandem Repeats 3 Examples of Restriciton Enzymes a E, H, -, ? b E, ?, - c E, H, - d ?, H, - 4 What Are The Cut Ends Called a Sticky Ends b Tacky Ends c Cut Ends d Slimy Ends 5 Where Do Restriciton Enzymes Cut DNA a Sticky Ends b Restriciton Site c Sugar Phosphate Bond d Nucleotides 6 Where are the shortest and longest fragments a Shortest at the bottom longest in the in the middle b Shortest at the top longest at the bottom c Shortest in the middle longest at the top d Shortest at the bottom longest at the top 7 What are the positive and negative electrode sides of the gel a Negative top and pos bottom b Negative bottom and pos top c Negative bottom and pos bottom d Negative top and pos top 8 Use of PCR a To delete DNA b To substitute DNA c To add DNA d To amplify (clone) DNA 9 PCR step 2 a Drop temperature to allow primers to anneal (attach) b Slightly elevate and then maintain temperature for a minute or two to allow the polymerase to elongate the new DNA strands c Heat samples to hottest temperature for a minute or so to separate double stranded template DNA 10 PCR step 3 a Drop temperature to allow primers to anneal (attach) b Slightly elevate and then maintain temperature for a minute or two to allow the polymerase to elongate the new DNA strands c Heat samples to hottest temperature for a minute or so to separate double stranded template DNA 11 PCR step 1 a Heat samples to hottest temperature for a minute or so to separate double stranded template DNA b Drop temperature to allow primers to anneal (attach) c Slightly elevate and then maintain temperature for a minute or two to allow the polymerase to elongate the new DNA strands d 12 What you need in the tube for PCR a Taq polymerase, sugar, water, buffer b Nucleotides, DNA templete strand, buffer, primase c Nucleotides, water, buffer, primase, DNA template strand d Taq polymerase, nucleotides, buffer, DNA template strand, primase 13 "Nickname" for Restriciton Enzymes a Molecular Scissors b Molecule Chopper c Molecular Cutter d Molecule Scissors 14 VNTRS Choose one or more answers a Long Nucleotide Length b Short Nucleotide Length c Variable Number Tandem Repeats d Short Tandem Repeats 15 Machine Name a Water Bath b Incubator c Oven d Thermo Cycler 16 What is the gel made of a Glucose b Sucrose c Agarose d Lactose 17 How can PCR's be used to identify individuals a Both males and females have Y and X chromosomes b Females don't have Y chromosomes c Males don't have X chromosomes d Males don't have Y chromosomes 18 Polymerase used in PCR a DNA polymerase b RNA polymerase c Taq Polymerase 19 Taq Polymerase a Lives in cold temperatures b Lives in the wateer c Lives in hot temperatures d Lives on land 20 Ligase a Joins newly inserted genes b Separates newly inserted genes c Joins newly deleted genes d Separates newly deleted genes 21 Bacteriophage a Virus from bacteria cells b Viruses that clone bacteria cells c Viruses that kill bacteria cells d Viruses that infect bacteria cells 22 Mircopipette a Used to dispense large amounts of liquid in mircoliters b Used to dispense small amounts of liquid in kiloliters c Used to dispense small amounts of liquid in microliters d Used to dispense large amount of liquids in kiloliters 23 Signifigance of DNA Charge a Negatively charged molecules move towards negative pole chamber b Negatively charged molecules move towards pos pole chamber c Negatively charge molecule don't move at all d Negatively charged molecules continuously move 24 Restriciton Enzymes a Proteins that cut DNA at specific sequences in bacteria b Proteins that cut DNA randomly in bacteria c Proteins that add DNA at specific sequences in bacteria d Proteins that add DNA randomly in bacteria 25 Purpose of dye Choose one or more answers a To visually track DNA b To see how to dye reacts to the substances c To weigh the sample down causing it to sink d For fun!

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Bio Study GuideVersión en línea

por Chloe Schneiderman

What are Flanking Sequences

STRS

Examples of Restriciton Enzymes

What Are The Cut Ends Called

Where Do Restriciton Enzymes Cut DNA

Where are the shortest and longest fragments

What are the positive and negative electrode sides of the gel

Use of PCR

PCR step 2

PCR step 3

PCR step 1

What you need in the tube for PCR

"Nickname" for Restriciton Enzymes

VNTRS

Machine Name

What is the gel made of

How can PCR's be used to identify individuals

Polymerase used in PCR

Taq Polymerase

Ligase

Bacteriophage

Mircopipette

Signifigance of DNA Charge

Restriciton Enzymes

Purpose of dye