POLYMERASE CHAIN REACTION Versión en línea CH 3 DYB30093 por munirah mihat 1 From where does Taq polymerase come? a Bacteria that live in hydrothermal vents/hot springs. b Cambodia c The rain forest d The CDC 2 What happens in the Denature step of PCR? a The DNA nucleotides are broken apart. b The base-pairing rules for DNA are reversed. c The double-stranded DNA is separated into two single strands of DNA. d The DNA is returned to its natural setting. 3 At what temperature does the Denature step of PCR occur? a 95° C b 50° C c 72° C d Any temperature 4 At what temperature does the Anneal step of PCR occur? a 95° C b 50° C c 72° C d Any temperature 5 At what temperature does the Extension step of PCR occur? a 95°C b 50-65°C c 72°C d Any temperature 6 What is the function of a primer? a To maintain the temperature of the PCR reaction. b To create DNA nucleotides. c To copy DNA. d To identify the particular region of DNA to be copied by PCR.