Relacionar Columnas Histology Chapter 1Versión en línea A fun and creative way to remember histology and its methods. por Taylor Shelton 1 what does clearing do? 2 What decreases the concentration of alcohol? 3 Two types of tissue preparation 4 Calcium ions in calcified bone 5 What does embedding do? 6 Peroxidase 7 What increases concentration of alcohol? 8 rehydration is used to do what? 9 staining 10 proteins attract what kind of dye? 11 Sectioning 12 cell structures attract what colored dye? 13 conventional light 14 polarizing 15 Name 5 fixatives 16 4 tissue types 17 what stains sugars? 18 phase contrast 19 Dehydration does what? 20 Sudan black stain 21 Two Tissue Components 22 Alkaline Phosphatase 23 Normarski 24 What does the fixation step do? In xylene to embed in tissue Paraffin-embedded tissue Frozen tissue Acid dye with positive charge. using alcohol to get out access fluid Epithelial Connective Muscular Nervous Dehydration Infiltrates tissue with solid medium to facilitate sectioning 10% Formalin 4% paraformaldehyde 70% Ethanol Methonal Glutaraldehyde and Osmium Tetroxide (EM) rehydration It preserves tissue structure Cut tissue into thin slices of about 10 micrometers. used to visualize structural features Cells Extracellular Matrix basic dye with negative charges PAS- Periodic Acid- Schiff reaction Remove paraffin
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