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Exam 2 BIO 274
Author :
amber smith
1.
What are psychophiles?
A
moderate loving (10-48ºC)
B
cold loving (-8-18ºC)
C
slight cold loving (0-31ºC)
2.
Which one likes (40-71ºC)
A
thermophiles
B
mesophiles
C
hyperthermophiles
3.
Psychotrophs prefer
A
0-31ºC
B
66-110ºC
C
-8-18ºC
4.
prefer 0-4 pH
A
acidophiles
B
neutrophiles
C
alkaliphiles
5.
alkaliphiles
A
9-11
B
6.5-7.5
C
0-4
6.
what does a non-halotolerant like?
A
2%-15% salinity
B
over 50% salinity
C
0-1.5% salinity
7.
which does not REQUIRE salt but will tolerate it?
A
falculative halophile
B
obligate halophile
C
non-halotolerant
8.
obligate aerobes...
A
need oxygen to metabolize
B
can use oxygen but doesn't have to
C
cannot use oxygen
9.
facultative aerobes...
A
absolutely cannot stand oxygen
B
can use oxygen or can switch
C
doesn't use it but can tolerate its presence
10.
which ones do not use any oxygen
A
aerotolerant anaerobe
B
microaerophiles
C
obligate anaerobe
11.
what does aerotolerate anaerobe mean
A
loves oxygen
B
doesn't use oxygen but tolerates it
C
can use oxygen in small amounts
12.
microaerophiles
A
can either use it or switch to something else
B
doesn't use oxygen but tolerates it
C
requires oxygen in small concentrations
13.
Quorum sensing is the word for...
A
sanitizing
B
when bacteria do not die from an antibiotic
C
cell communication
14.
what kind of culture media has all chemicals known
A
enrichment
B
chemically defined
C
TSA media plate
15.
what is a complex media
A
chemicals are unknown
B
media with added nutrients
C
selects what microbes grow
16.
when nutrients are added to a media it is then called a(n)
A
social media
B
complex media
C
enrichment media
17.
what is the benefit of adding nutrients to media?
A
so that the microbes don't get hungry
B
isolates organisms from a community
C
to help grow a diversity of organisms
18.
Selective media...
A
selects the growth for some but not all microbes
B
selects the best surviving organism and helps it reproduce
C
grows all microbes then picks one to die off
19.
Which media type causes changes to the media?
A
Diverse media
B
Differential media
C
Transitional Media
20.
How does bacteria reproduce
A
sexually
B
they must be scientifically bred
C
binary fission
21.
What does sterilization kill?
A
Endospores only
B
Thermophiles, harmful microbes, and pathogens
C
Endospores, thermophiles, pathogens, and microbes
22.
What is the difference between sterilization and commercial sterilization?
A
they are the same
B
the only endospore c. steriliz. can kill is C. botulinum, no other thermophiles
C
c. steriliz. can kill every plus is harmful to humans
23.
Which cleaning technique can only kill pathogens?
A
disinfection only
B
antisepsis only
C
disinfection and antisepsis
24.
What does sanitization kill?
A
all endospores and pathogens
B
harmful microbes only
C
just endospores
25.
If you used high-moist heat to kill an organism, what part(s) of the organism would you be harming?
A
DNA
B
enzymes
C
proteins (enzymes) and cell membranes
26.
How does pasteurization work?
A
freeze for 1 hour at 0ºC
B
boil at 72ºC for 15 minutes
C
bake at 100ºC for 30 mins
27.
What are the physical methods of microbial death?
A
High temps, low temps, desiccation, osmotic pressure, filtration and radiation
B
high temps, med. temps., atmospheric pressure, UV exposure, and filtration
C
freezing, boiling, radiation, injections, disinfection, and water submersion
28.
What organism structure do phenols attack?
A
nucleic acids
B
proteins
C
lipid membranes
29.
How do halogens attack microbes?
A
insert viral DNA and interrupt RNA transcription
B
alter cell membranes and effect protein synthesis
C
unravel DNA and melt membranes
30.
What is alcohol good for?
A
wound cleaning
B
a good friday night
C
degerming
31.
What does alcohol do to microbes?
A
Denatures proteins and disrupt cell membranes
B
disrupt viral envelopes
C
both of these are correct
32.
Why is alcohol not ideal for cleaning
A
70%-95% concentrate is needed for effectiveness
B
it has a strong odor
C
the CDC has reported it is less that 5% effective
33.
Define oligodynamic action
A
pairing with hydrogen to kill bacteria faster
B
this isn't a real term in the chapter
C
denature proteins by combining with sulfur groups
34.
what part of the cell do heavy metals attack?
A
proteins
B
nucleic acids
C
cell membranes
35.
What metal is in hand sanitizer?
A
Gold
B
Copper
C
Silver
36.
Why are peroxygens not used on open wounds?
A
Oxygen mixes with the blood and causes health issues
B
catalase in the cell can break it down and alter healing process
C
trick questions; it is used to clean wounds
37.
how to peroxygens attack cells?
A
denature proteins
B
inhibit protein synthesis
C
damage DNA
38.
What does acid-anionic sanitizer do?
A
disrupt plasma membrane
B
break up acid/base reactions
C
causes acid/base reactions
39.
Which of the following specifically affect Gram pos. bacteria?
A
hydrogen peroxide
B
hand sanitizer
C
Quaternary ammonia compounds (QUATS)
40.
If antibiotics fight bacteria, why are they made from bacteria?
A
to attack other bacterial species
B
to self attack
C
it is not yet known
41.
If an antibiotic can be used to treat 20 different infections, it has a(n)...
A
narrow spectrum
B
broad spectrum
C
wide spectrum
42.
Which isn't one of the mechanisms of antimicrobial actions
A
Synthesis of metabolite inhibition
B
injury to plasma membrane
C
blocking entry
43.
What does penicillin do to fight infection?
A
disrupts DNA translation
B
prevents peptidoglycan synthesis
C
denatures proteins
44.
What does bacitracin do to attack cells?
A
interferes with synthesis of linear strands of peptidoglycan
B
inhibits cell wall synthesis
C
inhibits proteins synthesis
45.
Which of the following inhibits cell wall synthesis?
A
vancomycin
B
cephalosporins
C
bacitracin
46.
Ethromyocin does what?
A
kills pathogens
B
blocks tunnel in ribosome structure and inhibits growing polypeptides
C
mutates DNA
47.
What is unique about quiolones?
A
inhibits DNA gyrase and causes DNA supercoils to unravel
B
it only affects Gram neg. cells
C
it doesn't work against bacteria, only pathogens
48.
Rifampin does the following...
A
inhibits cell membrane synthesis
B
binds to host cells and injects viral DNA
C
inhibits RNA synthesis
49.
what is rifampin good for treating?
A
Tuberculosis
B
COVID
C
Influenza
50.
Which mechanism binds to active site and blocks substrate?
A
ethromyocin
B
sulfonamides
C
isoniazid
51.
True of False; Miconazole, Echinoandins, and Flucytocine are all Antifungal drugs
A
True
B
False
C
It depends in the type of fungus
52.
How does Acycolvir (Antiviral drug) work?
A
eats away at cell membrane
B
incorporates fake nucleotide into DNA to block replication
C
burns & denatures proteins/enzymes
53.
The anti fungal drug Flucytocine works by...
A
mutating DNA
B
stopping DNA replication
C
converting into uracil to make RNA inactive
54.
Which cell structure does the anti fungal drug Echinocandin attack?
A
cell wall
B
nucleus
C
ribosomes
55.
Antifungal drug Miconazole is specific to the...
A
nucleic acids
B
cell membrane
C
flagella
56.
Which antiviral dug contains a two step process?
A
Telaprevir
B
Niratrelvir-ritonavir (Paxlovid)
C
Remdesvir
57.
Are anti protozoan drugs affective against Malaria?
A
Yes
B
No
C
Only when used in specific concentrations
58.
What does Niclosamide protect against? How does it work?
A
tapeworms; blocks ATP production
B
blood flukes; neuromuscular block
C
roundworms; neuromuscular block
59.
What does Praziquantel protect against and how does it work?
A
roundworms; neuromuscular block
B
tapeworms; blocks ATP production
C
flukes; alters plasma membrane permeability
60.
Which Antihelminthic drug blocks neuromuscular activity and treat roundworms?
A
Niclosamide
B
Pyrantel pamote
C
Praziquantel
61.
Which reaction type adds water to BREAK bonds
A
Hydrolysis
B
Dehydration synthesis
C
Anabolic
62.
What is Dehydration synthesis?
A
adding water to break bonds
B
amount of water taken away to begin a reaction
C
removal of water to form bonds
63.
Define collision theory
A
random collisions occur because molecules are constantly moving
B
molecules are attracted to other molecules and "chase" them
C
binding to another molecule because of the molecular anatomy
64.
Where does a substrate bind?
A
Substrate site
B
Allosteric site
C
Active site
65.
Inhibitor binding occurs in the...
A
Allosteric site
B
Inhibitor site
C
Active site
66.
True or false: Enzymes can be reused
A
False
B
True
C
there is not enough information to make a definitive choice
67.
How do non-competitive inhibitors work?
A
binds to allosteric site-active site changes shape-substrate cannot bind
B
substance binds to active site to block inhibitor
C
substance binds to allosteric site and blocks inhibitor
68.
What is the reduced form of NAD+?
A
NADP
B
NADH
C
NADPH
69.
The oxidized form of FADH2 is...
A
FAD+
B
NADPH
C
NADH
70.
Which is the correct pairing?
A
NAD+----NADPH
B
NADP+----NADPH
C
FAD+----FADPH
71.
Which of the following is NOT a way to make ATP?
A
Glycolic phosphorylation
B
Oxidative phosphorylation
C
Photophosphorylation
72.
Which form of making ATP involves passing phosphate to ADP after a series of oxidative steps?
A
substrate level phosphorylation
B
photophosphorylation
C
oxidative phosphorylation